Potential therapeutic phytochemicals from Ocimum basilicum for the prevention and management of neurodegenerative diseases
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1
Universiti Kebangsaan Malaysia , Drug and Herbal Research Centre, Faculty of Pharmacy, Malaysia
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2
Universiti Kebangsaan Malaysia, Faculty of Health Sciences, Malaysia
Ocimum basilicum Linn., also known as sweet basil, is a member of the Lamiaceae family. It is used in traditional medicine to treat several ailments principally of the digestive and interestingly of the nervous system possibly due to its antioxidant properties. The neuroprotective properties of this plant have not been extensively studied which warrants for further investigation of its putative effect. In the present study, rosmarinic acid content was quantified in crude hydroethanolic extracts of the flower, leaf and stem using HPTLC. The crude extract of Ocimum basilicum leaf was sequentially fractionated with hexane, ethyl acetate and ethanol to determine their rosmarinic acid and total phenolic content. In addition, neuroprotective effects of phenolic-rich fractions of the leaf on H2O2-induced oxidative stress on SK-N-SH neuroblastoma cells were evaluated. Protective effects against memory deficits in a pharmacological model of Alzheimer’s disease were studied in rats. It was found that the flower contained the highest rosmarinic acid followed by leaf and stem. Ethyl acetate fraction of the leaf contained the highest rosmarinic acid followed by ethanol and hexane fraction. The highest amount of total phenolic-rich fractions was found in ethanol>ethyl acetate>hexane fractions. Pretreatment with ethyl acetate fraction attenuated cell death induced by H2O2 to a greater extent than ethanol fraction and crude extract. Pretreatment with the leaf extract at 200 mg/kg protected the rats from scopolamine-induced memory impairment. These results suggest that Ocimum basilicum possesses neuroprotective actions possibly associated with its antioxidant properties.
Keywords:
Oxidative Stress,
Neuroprotection,
neurodegeneration,
antioxidant,
Sweet basil
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Symposium 7: New Insights on Oxidative Stress in Neurodegenerative Diseases and Therapeutic Potential of Anti- Oxidants as a Drug for the Pre-Emptive Medicine
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Azmi
N,
Mohd-Zahid
MH,
Jalil
J,
Chan
KM,
Yaakob
NS and
Tee
HH
(2016). Potential therapeutic phytochemicals from Ocimum basilicum for the prevention and management of neurodegenerative diseases.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00032
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Received:
26 Jul 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Norazrina Azmi, Universiti Kebangsaan Malaysia, Drug and Herbal Research Centre, Faculty of Pharmacy, Kuala Lumpur, Wilayah Persekutuan (KL), Malaysia, azrina.azmi@ukm.edu.my